Small Bowel Biopsy
A definitive diagnosis of CSID can be made by demonstrating the absence of sucrase-isomaltase in a small intestinal biopsy of the distal duodenum or of the proximal jejunum.
A biopsy may be obtained either with a Crosby capsule placed in the jejunum or with the endoscope in the distal duodenum or proximal jejunum.
Method:
Mucosal tissue is homogenized and incubated with the appropriate disaccharide substrate. Enzyme hydrolysis results in the formation of glucose. A quantitative measurement is then done on the glucose yielded.Specimen Requirements and Shipping:
Approximately 5 mg of small bowel should be collected through endoscopy for the complete enzyme analysis.
The tissue should be placed on the wall of a small, tightly capped, plastic tube appropriately labeled with the patient name, date of birth, ID number, date specimen collected and referring physician name. A 1.5 mL microtube works well. Please do not place the tissue on gauze, filter paper, or use any type of support medium.
Freeze immediately and keep frozen for shipping on dry ice.
Package according to regulations regarding shipment of infectious material and pack with enough dry ice to last 48 hours. Ship overnight by appropriate courier (Airborne / Fed Ex).
Laboratories are closed weekends. When shipping, please ship overnight and ensure a weekday delivery.
Where to Ship:
The following laboratories perform disaccharidase analyses:
1348 N Forest Rd
Williamsville, New York 14221
(716) 639-0443
For full instructions Joli Diagnostic PDF
Women’s & Children’s Hospital of Buffalo
Gastroenterology Lab
219 Bryant St,
Buffalo, NY 14222
(716) 878-7652 (ask for Bridget, Shari or Cindy)
2211 Michigan Avenue
Santa Monica, CA 90404-3900
Toll free:1-800-421-7110, ext. 1
Phone: (310) 828-6543, ext. 1
Fax: (310) 828-8153
Department of Pediatrics
Nita Salzman MD, PhD
Medical College of Wisconsin
MACC Fund Research Center – Room 4048
8701 Watertown Plank Road
Milwaukee, WI 53226
Phone: (414) 456-4166
Fax: (414) 456-6686
Specimen collection:Small Bowel Biopsy Place in small plastic tubes (no saline or gauze). Must be tightly capped and stored at -70°C.
Please send samples in approximately 5 pounds of dry ice express mail, preferably at the beginning of the week (no later than Wednesday morning), with a brief patient history to the above address. Results will be sent out no more than 3 working days after receipt.
To expedite delivery, Dr. Nita Salzman and phone number (414) 456-4166 should appear on the package address label. We are not licensed to bill patients or their insurance; we can only bill the referring institution. Please call ahead for holiday hours. The laboratory is closed on weekends.
